A novel method for early detection of MIC value – Broth dilution using indicator solution versus agar dilution: an original article

Introduction: The adequate protocol for treatment of an infection is often determined on the basis minimum inhibitory concentration (MIC) causative organism. Traditional methods (agar dilution, microbroth and gradient diffusion) are labour intensive time consuming (they usually take over 48 hours to report results). On other hand, automated systems (VITEK™, Phoenix™, MicroScan WalkAway™) rapid MIC detection (using dielectrophoresis (DEP), magnetic bead rotation sensors microfluidic incubation) require expensive instruments. This study aimed develop a method with ability applied resource limited setting. Methods: Agar dilution novel broth (containing indicator solution) were simultaneously performed using amikacin, ceftriaxone, piperacillin-tazobactam, imipenem, cefoxitin azithromycin. Results: Isolates Escherichia coli, Enterobacter spp, Klebsiella Staphylococcus aureus, Proteus mirabilis, Acinetobacter spp Pseudomonas used. values Enterobacteriaceae S. aureus isolates each antibiotic obtained within 4 5 by method. corresponded shown following day agar Conclusion: Broth solution effective in determination cephalosporins, penicillin, carbapenems, cephamycin, aminoglycosides macrolides most aureus. Unfortunately this did not work non-fermenter group organisms like as their results could be before 24 hours. saving, relatively inexpensive applicable settings.